Collaborators
Among the group of users, we have active projects in:
- cardiac metabolism (Dr. Rong Tian)
- skeletal muscle metabolism and energetics (Drs. David Marcinek and Martin Kushmerick)
- aged muscle studies (Drs. David Marcinek and Peter Rabinovitch)
- mouse tumor biology and molecular imaging (Dr. Donghoon Lee)
- mouse traumatic brain injury and Alzheimer’s imaging (Dr. Donna Cross)
Please contact us to find out how we can help you with your research.
Examples of Images Generated from the NMR Facility
T1-weighted transverse cross-sections displaying mouse anatomy from pelvis to head as well as a coronal image of a mouse brain.
Images acquired by Peter Chiarelli and Richard Revia.
T2-weighted coronal and sagittal images (left and middle columns, respectively) as well as T2*-weighted images (right column) of mice brains 1.5 hours (top row) and 9 hours (bottom row) after injection of iron-oxide nanoparticles.
Images acquired by Peter Chiarelli and Richard Revia.
Fractional Anisotropy (FA) images acquired with the Diffusion Tensor Imaging (DTI) sequence show that 3xTg-AD mice have decreased white matter integrity at 10-12 weeks.
Images acquired by Donna Cross, Marcella Cline, Bradley Neel, and Greg Garwin.
T1-weighted (left) and T2-weighted images of tumor (arrows) in transgenic mouse model of pancreatic ductal adenocarcinoma.
Images acquired by Donghoon Lee and Joshua Park.
Multi-parametric imaging of pancreatic ductal adenocarcinoma in a transgenic KPC mouse (A, B, C, and D) and in a xenograft tumor mouse (E, F, G, H, and I). A and E: T1-weighted. B and F: T2-weighted. C and G: Quantitative T2 map. D and H: Apparent diffusion coefficient map. I: Magnetization transfer image.
Images acquired by Donghoon Lee and Joshua Park.
Images of NIH Swiss mice treated (right) and untreated (left) with the chemical NTCU for 6 months. Lesions observed in the MRI images in treated animals correspond to atypical hyperplasia and squamous cell carcinoma in the histological analysis of the lung.
Images acquired by Laura Riolobos Santamaria.
Publications
The 14T HRIM Facility was utilized to complete studies in the following publications:
2015
Kolwicz, S.C., Liu, L., Goldberg, I.J., and Tian, R. Enhancing cardiac triacylglycerol metabolism improves recovery from ischemic stress. Diabetes, 64(8):2817-27, 2015.
2014
Karamanlidis, G., Garcia-Menendez, L., Kolwicz, S.C., Lee, C.F., and Tian, R. Promoting PGC1α-driven mitochondrial biogenesis is detrimental in pressure overloaded mouse hearts. American Journal of Physiology Heart and Circulatory Physiology, 307(9):H1307-16, 2014.
Kim, M., Hunter, R.W., Garcia-Menendez, L., Gong, G., Yang, Y.Y., Kolwicz, S.C., Xu, J., Wang, W., Sakamoto, K., Tian, R. Mutation in the γ2-subunit of AMPK stimulates cardiomyocyte proliferation and hypertrophy independent of glycogen storage. Circulation Research, 114(6):966-75, 2014.
Yu Q, Lee CF, Wang W, Karamanlidis G, Kuroda J, Matsushima S, Sadoshima J, Tian R. Elimination of NADPH oxidase activity promotes reductive stress and sensitizes the heart to ischemic injury. J Am Heart Assoc, 3(1):e000555, 2014.
2013
Karamanlidis, G., Lee, C.F., Garcia-Menendez, L., Kolwicz, S.C., Suthammarak, W., Gong, G., Sedensky, M.M., Morgan, P.G., Wang, W., and Tian, R. Mitochondrial complex I deficiency increases protein acetylation and accelerates heart failure. Cell Metabolism, 18(2):239-250, 2013.
Garcia-Menendez, L., Karamanlidis, G., Kolwicz S.C., and Tian R. Substrain specific response to cardiac pressure overload in C57BL/6 mice. American Journal of Physiology Heart and Circulatory Physiology, 305: H397-H402, 2013.
Nowakowski, S.G.* and Kolwicz, S.C.*, Korte, F.S., Luo, Z., Robinson-Hamm, J.N., Page, J.L., Brozovich, F.V., Weiss, R.S., Tian, R., Murry, C.E., and Regnier, M. Transgenic overexpression of ribonucleotide reductase improves cardiac performance. Proceedings of the National Academy of the Sciences USA, 110(15): 6187-6192, 2013. *Equal contribution for first author
We kindly ask all users to acknowledge the facility grant in their publications using the following statement: This work was supported by the NIH Biomedical Research Support Shared Instrumentation Grant (S10RR029021 to 14T HRIM Facility).