Tie2 and Endothelial cells :
- HUVECs western blot protocol
- Tie2-specific immunofluorescence staining protocol (Blair)
- Conjugation Syptag to Spycatcher
- Size Exclusion Chromatography S6 (SLU Shally)
- Tube formation assay (Blair)
- Blair‘s IF and WB staining antibody dilutions
- siRNA knockout of Tie2/TEK
- EC differentiation protocol (Devon)
- EC differentiation – day 5 harvest (Devon)
- EC Induction Media Calculator (Devon)
- EC differentiation protocol (Devon)
- EC differentiation – day 5 harvest (Devon)
- EC Induction Media Calculator (Devon)
- EGM2 (Growth medium for HUVEC cells) – ECGS Extraction Protocol (Cheryl Chan)
Cardiomyocytes :
- Cardiac Differentiation (Elisa clark)
- Cardio-fibroblast differentiation (Shally)
- Lactate Selection and Replating CMs (Elisa Clark)
- Making BSA and AA for Cardiac differentiation (Elisa Clark)
Gene editing:
- CRIPSRCas9
- pGEM cloning
- In_Vitro_Transcription of guide RNA’s
- Go_Taq_and_Q5_PCR_amplification_protocols
- Gibson Assembly® Protocol (E5510)
Transfection :
Plasmids :
- Cloning custom sgRNAs into Zhang lab
- Single-temperature Double Digest
- T4 ligation and Gibson assembly protocol
- Q5 self annealing PCR
Staining :
- Staining_protocol (Damien)
- Intracellular and extracellular staining (Elisa Clark)
- Confocal Imaging
- AP staining
Bacteria Culture and Transformation:
qPCR:
- Making cDNA
- Trizol Extraction
- Trizol RNA Extraction And RTPCR Protocol
Other :
- Max Gordon‘s PCR, Western Blot, and Cell culture protocols
- Western Blot – Liquid Transfer (Cheryl)
- FlowCytometry Protocols
- Making Matrigel Plates protocol
- Splitting cells with Feeder Layer
- Plating MEFs
- MiMaC Transduction of hiPSC-CMs (Elisa Clark)
- Generation_of_iPSC_from_fibroblasts_Julie_June_2013
- AAdams’ ES Culture Protocols_updated October09
- Co-IP for HIF protein Protocol (Matthew’s)
- XFe96 protocol (Yen Lim)
- Protein Production Materials and Methods (YCL)