* Indicates equal contribution.
2021-2024
Dunnington, E.L.;* Wong, B.S.;* Fu, D. Innovative Approaches for Drug Discovery: Quantifying Drug Distribution and Response with Raman Imaging. Analytical Chemistry 2024. doi: 10.1021/acs.analchem.4c01413.
Xu, F.X.; Ioannou, G.N.; Lee, S.P.; Savard, C.; Horn, C.L.; Fu, D. Discrimination of Lipid Composition and Cellular Localization in Human Liver Tissues by Stimulated Raman Scattering Microscopy. J. Biomed. Opt. 2024, 29 (1), 016008. doi: 10.1117/1.JBO.29.1.016008.
Wong, B.S.;* Dunnington, E.L.;* Wu, R.; Kim, J.I.; Hu, K.; Ro, T. H.; Fu, D. Facilitated Transport of EGFR Inhibitors Plays an Important Role in Their Cellular Uptake. Analytical Chemistry 2024, 96 (4), 1547-1555. doi: 10.1021/acs.analchem.3c04242.
Xu, F.X.; Wu, R.; Hu, K.; Fu, D. Measuring Drug Response with Single-Cell Growth Rate Quantification. Analytical Chemistry 2023, 95 (49), 18114-18121. doi: 10.1021/acs.analchem.3c03434
Wong, B.; Zhao, X.; Su, Y.; Ouyang, H.; Rhodes, T.; Xu, W.; Xi, H.; Fu, D. Characterizing Silicone Oil-Induced Protein Aggregation with Stimulated Raman Scattering Imaging. Mol. Pharmaceutics 2023, 20 (8), 4268-4276. doi: 10.1021/acs.molpharmaceut.3c00391
Xu, F.X.; Rathbone, E.G.; Fu, D. Simultaneous Dual-Band Hyperspectral Stimulated Raman Scattering Microscopy with Femtosecond Optical Parametric Oscillators. The Journal of Physical Chemistry B 2023, 127 (10), 2187-97. doi.org/10.1021/acs.jpcb.2c09105
Figueroa, B.; Xu, F. X.; Hu, R.; Men, S.; Fu, D. Quantitative Imaging of Intracellular Density with Ratiometric Stimulated Raman Scattering Microscopy. The Journal of Physical Chemistry B 2022, 126 (39), 7595-7603, doi:10.1021/acs.jpcb.2c04355
Manifold, B.; Fu, D. Quantitative Stimulated Raman Scattering Microscopy – Promises and Pitfalls. Annual Reviews in Analytical Chemistry 2022, 15, 269-289. doi: 10.1146/annurev-anchem-061020-015110
Oh, S.; Lee, C.; Yang, W.; Li, A.; Ran, C.; Yin, W.; Tabin, C.J.; Fu, D.; Xie, X.S.; Kirschner, M.W. Protein and lipid mass concentration measurement in tissues by stimulated Raman scattering microscopy. Proceedings of the National Academy of Sciences 2022, 119 (17) e2117938119. doi: 10.1073/pnas.2117938119.
Francis, A.T.; Manifold, B.; Carlson, E.C.; Hu, R.; Hill, A.H.; Men, S.; Fu, D. In vivo simultaneous nonlinear absorption Raman and fluorescence (SNARF) imaging of mouse brain cortical structures. Communications Biology 2022, 5 (1), 222. doi: 10.1038/s42003-022-03166-6.
Shin K.S.; Men, S.; Wong, A.; Cobb-Bruno, C.; Chen, E.Y.; Fu, D. Quantitative Chemical Imaging of Bone Tissue for Intraoperative and Diagnostic Applications. Analytical Chemistry 2022, 94 (9), 3791-3799. doi: 10.1021/acs.analchem.1c04354.
Espinoza, R.;* Wong, B.;* Fu, D. Real-time, Two-color Stimulated Raman Scattering Imaging of Mouse Brain for Tissue Diagnosis. Journal of Visualized Expriments 2022, 180, 10.3791/63484. doi: 10.3791/63484.
Manifold, B.; Men, S.; Hu, R.; Fu, D. A versatile deep learning architecture for classification and label-free prediction of hyperspectral images. Nature Machine Intelligence 2021, 3, 306-315. doi: 10.1038/s42256-021-00309-y.
Barlow, S.T.; Figueroa, B.; Fu, D.; Zhang, B. Membrane Tension Modifies Redox Loading and Release in Single Liposome Electroanalysis. Analytical Chemistry 2021, 93 (8), 3876–82. doi: 10.1021/acs.analchem.0c04536.
2016-2020
Francis, A.; Shears, M.J.; Murphy, S.C.; Fu, D. Direct Quantification of Single Red Blood Cell Hemoglobin Concentration with Multiphoton Microscopy. Analytical Chemistry 2020, 92 (18), 12235-41. doi: 10.1021/acs.analchem.0c01609
Figueroa, B.; Hu, R., Rayner, S.G., Zheng, Y., Fu, D. Real-Time Microscale Temperature Imaging by Stimulated Raman Scattering. Journal of Physical Chemistry Letters 2020, 11 (17), 7083-9. doi: 10.1021/acs.jpclett.0c02029
Shin, K.S.; Laohajaratsang, M.; Men, S.; Figueroa, B.; Dintzis, S.M.; Fu, D. Quantitative chemical imaging of breast calcifications in association with neoplastic processes. Theranostics 2020, 10 (13), 5865-78. doi:10.7150/thno.43325
Hill, A.;* Manifold, B.;* Fu, D. Tissue imaging depth limit of stimulated Raman scattering microscopy. Biomedical Optics Express 2020, 11 (2), 762-774. doi: 10.1364/BOE.382396
Shin, K.; Francis, A.; Hill, A.; Laohajaratsang, M.; Cimino, P.; Latimer, C.; Gonzalez-Cuyar, L.; Sekhar, L.; Juric-Sekhar, G.; Fu, D. Intraoperative assessment of skull base tumors using stimulated Raman scattering microscopy. Scientific Reports 2019, 9, 20392. doi: s41598-019-56932-8
Hill, A.; Munger, E.; Francis, A.; Manifold, B.; Fu, D. Frequency Modulation Stimulated Raman Scattering Microscopy through Polarization Encoding. Journal of Physical Chemistry B 2019, 123 (40), 8397-8404. doi: 10.1021/acs.jpcb.9b07075
Francis, A.F.; Berry, K.; Thomas, E.C.; Hill, A.H.; Fu, D. In vitro quantification of single red blood cell oxygen saturation by femtosecond transient absorption microscopy. Journal of Physical Chemistry Letters 2019, 10 (12), 3312-3317. doi: 10.1021/acs.jpclett.9b01116
Manifold, B.; Thomas, E.C.; Francis, A.; Hill, A.; Fu, D. Denoising of stimulated Raman scattering microscopy images via deep learning. Biomedical Optics Express 2019, 10 (8), 3860-3874. doi: 10.1364/BOE.10.003860
Figueroa, B.; Nguyen, T.; Sotthivirat, S.; Xu, W.; Rhodes, T.; Lamm, M.S.; Smith, R.L.; John, C.T.; Su, Y.; Fu, D. Detecting and Quantifying Microscale Chemical Reactions in Pharmaceutical Tablets by Stimulated Raman Scattering Microscopy. Analytical Chemistry 2019, 91 (10), 6894-6901. doi: 10.1021/acs.analchem.9b01269
Molina, R.M.; Konduru N.V.; Queiroz P.M.; Figueroa B.; Fu, D.; Ma-Hock, L.; Groeters, S.; Schaudien D.; Brain, J.D. Fate of Barium Sulfate Nanoparticles Deposited in the Lungs of Rats. Scientific Reports 2019, 9, 8163. doi: s41598-019-44551-2
Hill, A.; Fu, D. Cellular Imaging Using Stimulated Raman Scattering Microscopy. Analytical Chemistry 2019, 91 (15), 9333-9342. doi: 10.1021/acs.analchem.9b02095
Francis, A.; Nguyen, T.T.; Lamm, M.S.; Teller, R.; Forster, S.P.; Xu, W.; Rhodes, T.; Smith, R.L.; Kuiper, J.; Su, Y.; Fu, D. In Situ Stimulated Raman Scattering (SRS) Microscopy Study of the Dissolution of Sustained-Release Implant Formulation. Molecular Pharmaceutics 2018, 15 (12), 5793–5801. doi: 10.1021/acs.molpharmaceut.8b00965
Figueroa, B.; Fu, W.; Nguyen, T.; Shin, K.; Manifold, B.; Wise, F.; Fu, D. Broadband hyperspectral stimulated Raman scattering microscopy with a parabolic fiber amplifier source. Biomedical Optics Express 2018, 9 (12), 6116-6131. doi: 10.1364/BOE.9.006116
Francis, A; Berry, K.; Chen, Y.; Figueroa, B.; Fu, D. Label-free Pathology by Spectrally Sliced Femtosecond Stimulated Raman Scattering (SRS) Microscopy. PLoS One 2017, 12 (5), e0178750. doi: 10.1371/journal.pone.0178750
Fu, D. Quantitative Chemical Imaging with Stimulated Raman Scattering Microscopy. Current Opinions in Chemical Biology 2017, 39, 24-31. doi: 10.1016/j.cbpa.2017.05.002
Figueroa, B.; Chen, Y.; Berry, K.; Francis, A.; Fu, D. Label-free chemical imaging of latent fingerprints with stimulated Raman scattering microscopy. Analytical Chemistry 2017, 89 (8), 4468–4473. doi: 10.1021/acs.analchem.6b04213
Fu, D.; Yang, W.; Xie, X.S. Label-free Imaging of Neurotransmitter Acetylcholine at Neuromuscular Junctions with Stimulated Raman Scattering. Journal of the American Chemical Society 2017, 139 (2), 583–586. doi: 10.1021/jacs.6b10727
Villareal, V. A.; Fu, D.; Costello, D.A.; Xie, X.S.; Yang, P.L. Hepatitis C Virus Selectively Alters the Intracellular Localization of Desmosterol. ACS Chemical Biology 2016, 11 (7), 1827–1833. doi: 10.1021/acschembio.6b00324
2011-2015
Ji, M.; Lewis, S.; Camelo-Piragua, S.; Ramkissoon, S.H.; Snuderl, M.; Venneti, S.; Fisher-Hubbard, A.; Garrard, M.; Fu, D.; Wang, A. C.; Heth, J. A.; Maher, C. O.; Sanai, N.; Johnson, T.D.; Freudiger, C.W.; Sagher, O.; Xie, X.S.; Orringer, D.A. Detection of human brain tumor infiltration with quantitative stimulated Raman scattering microscopy. Science Translational Medicine 2015, 7 (309), 309ra163.
Lu, F..; Basu, S.; Igras, V.; Hoang, M.P.; Ji, M.; Fu, D.; Holtom, G.R.; Neel, V.A.; Freudiger, C.W.; Fisher, D.E.; Xie, X.S. Label-free DNA imaging in vivo with stimulated Raman scattering microscopy. Proceedings of the National Academy of Sciences USA 2015, 112 (37), 11624–11629.
Fu, D.; Zhou, J.; Zhu, W.; Manley, P.W.; Wang, Y.; Hood, T.; Wylie, A.; Xie, X.S. Imaging the intracellular distribution of tyrosine kinase inhibitors in living cells with quantitative hyperspectral stimulated Raman scattering. Nature Chemistry 2014, 6 (7), 614–622. doi: 10.1038/nchem.1961
Fu, D.; Yu, Y.; Folick, A.; Currie, E.; Farese, R.V.; Tsai, T.-H.; Xie, X. S.; Wang, M. C. In Vivo Metabolic Fingerprinting of neutral lipids with hyperspectral stimulated Raman scattering microscopy. Journal of the American Chemical Society 2014, 136 (24), 8820–8828. doi: 10.1021/ja504199s
Fu, D.; Xie, X.S. Reliable cell segmentation based on spectral phasor analysis of hyperspectral stimulated Raman scattering imaging data. Analytical Chemistry 2014, 86 (9), 4115–4119. doi: 10.1021/ac500014b
Kong, L.; Ji, M.B.; Holtom, G.R.; Fu, D.; Freudiger, C.W.; Xie, X.S. Multicolor stimulated Raman scattering microscopy with a rapidly tunable optical parametric oscillator. Optics Letters 2013, 38 (2), 145–147. doi: 10.1364/OL.38.000145
Fu, D.; Holtom, G.; Freudiger, C.; Zhang, X.; Xie, X.S. Hyperspectral imaging with stimulated Raman scattering by chirped femtosecond lasers. Journal of Physical Chemistry B 2013, 117 (16), 4634–4640. doi: 10.1021/jp308938t
Zhang, X.; Roeffaers, M.B.J.; Basu, S.; Daniele, J.R.; Fu, D.; Freudiger, C.W.; Holtom, G.R.; Xie, X.S. Label-free live-cell imaging of nucleic acids using stimulated Raman scattering microscopy. ChemPhysChem 2012, 13 (4), 1054–1059. doi: 10.1002/cphc.201100890
Yu, Z.; Chen, T.; Zhang, X.; Fu, D.; Liao, X.; Shen, J.; Liu, X.; Zhang, B.; Xie, X.S.; Su, X.; Chen, J.; Huang, Y. Label-free chemical imaging in vivo: three-dimensional non-invasive microscopic observation of amphioxus notochord through stimulated Raman scattering. Chemical Science 2012, 3 (8), 2646–2654. doi: 10.1039/C2SC20316B
Oh, S.; Fang-Yen, C.; Choi, W.; Yaqoob, Z.; Fu, D.; Park, Y.; Dassari, R.R.; Feld, M. S. Label-free imaging of membrane potential using membrane electromotility. Biophysical Journal 2012, 103 (1), 11–18. doi: 0.1016/j.bpj.2012.05.020
Lu, F.; Ji, M.; Fu, D.; Ni, X.; Freudiger, C.W.; Holtom, G.; Xie, X.S. Multicolor stimulated Raman scattering microscopy. Molecular Physics 2012, 110 (15-16), 1927–1932. doi: 10.1080/00268976.2012.695028
Lefrancois, S.; Fu, D.; Holtom, G.R.; Kong, L.J.; Wadsworth, W.J.; Schneider, P.; Herda, R.; Zach, A.; Xie, X.S.; Wise, F.W. Fiber four-wave mixing source for coherent anti-Stokes Raman scattering microscopy. Optics Letters 2012, 37 (10), 1652–1654. doi: 10.1364/OL.37.001652
Fu, D.; Lu, F.; Zhang, X.; Freudiger, C.; Pernik, D.R.; Holtom, G.; Xie, X.S. Quantitative chemical imaging with multiplex stimulated Raman scattering microscopy. Journal of the American Chemical Society 2012, 134 (8), 3623–3626. doi: 10.1021/ja210081h
Yaqoob, Z.; Yamauchi, T.; Choi, W.; Fu, D.; Dasari, R.R.; Feld, M. S. Single-shot full-field reflection phase microscopy. Optics Express 2011, 19 (8), 7587–7595. doi: 10.1364/OE.19.007587
2006-2010
Fu, D.; Oh, S.; Choi, W.; Yamauchi, T.; Dorn, A.; Yaqoob, Z.; Dasari, R.R.; Feld, M.S. Quantitative DIC microscopy using an off-axis self-interference approach. Optics Letters 2010, 35 (14), 2370–2372. doi: 10.1364/OL.35.002370
Fu, D.; Choi, W.; Sung, Y.; Yaqoob, Z.; Dasari, R.R.; Feld, M.S. Quantitative dispersion microscopy. Biomedical Optics Express 2010, 1 (2), 347–353. doi: 10.1364/BOE.1.000347
Ye, T.; Fu, D.; Warren, W.S. Nonlinear absorption microscopy. Photochemistry and Photobiology 2009, 85 (3), 631–645. doi: 10.1111/j.1751-1097.2008.00514.x
Fu, D.; Choi, W.; Sung, Y.; Oh, S.; Yaqoob, Z.; Park, Y.; Dasari, R.R.; Feld, M.S. Ultraviolet refractometry using field-based light scattering spectroscopy. Optics Express 2009, 17 (21), 18878–18886. doi: 10.1364/OE.17.018878
Austin, R.H.; Xie, A.; Fu, D.; Warren, W.; Redlich, B.; van der Meer, L. Tilting after Dutch windmills: probably no long-lived Davydov solitons in proteins. Journal of Biological Physics 2009, 35 (1), 91–101. doi: 10.1007/s10867-009-9130-7
Fu, D.; Ye, T.; Matthews, T.E.; Grichnik, J.; Hong, L.; Simon, J.D.; Warren, W.S. Probing skin pigmentation changes with transient absorption imaging of eumelanin and pheomelanin. Journal of Biomedical Optics 2008, 13 (5), 054036. doi: 10.1117/1.2976424
Fu, D.; Matthews, T.E.; Ye, T.; Piletic, I.R.; Warren, W.S. Label-free in vivo optical imaging of microvasculature and oxygenation level. Journal of Biomedical Optics 2008, 13 (4), 040503. doi: 10.1117/1.2968260
Fu, D.; Ye, T.; Matthews, T.E.; Yurtsever, G.; Warren, W.S. Two-color, two-photon, and excited-state absorption microscopy. Journal of Biomedical Optics 2007, 12 (5), 054004. doi: 10.1117/1.2780173
Fu, D.; Ye, T.; Matthews, T.E.; Chen, B. J.; Yurtserver, G.; Warren, W.S. High-resolution in vivo imaging of blood vessels without labeling. Optics Letters 2007, 32 (18), 2641–2643. doi: 10.1364/OL.32.002641
2003-2005
Austin, R.H.; Xie, A.; van der Meer, L.; Redlich, B.; Lindgard, P.A.; Frauenfelder, H.; Fu, D. Picosecond thermometer in the amide I band of myoglobin. Physical Review Letters 2005, 94 (12), 128101. doi: 10.1103/PhysRevLett.94.128101
Fu, D.; Jing, X. Chromaticity simulation for the influence of the blue phosphor spectrum to FED properties, Chinese Journal of Luminescence 2004, 25 (3), 320–324.
Liu, T.; Fu, D.; Gao, S.; Zhang, Y.; Sun, H.; Su, G.; Liu, Y. An azide-bridged homospin single-chain magnet: [Co(2,2′-bithiazoline)(N3)2]n. Journal of the American Chemical Society 2003, 125 (46), 13976-13977. doi: 10.1021/ja0380751