Collaboration between members of the different labs of the center is encouraged by offering shared, state-of-the-art equipment along with guidance from highly-trained personnel.
Confocal Microscope
The advances of confocal microscopy allow for the generation of high resolution, quality images of both fixed and living cells. The use of spatial filtering techniques, such as the confocal aperture to eliminate out of focus light, and the use of laser scanning renders three-dimensional, accurate images.
If you wish to access the Confocal Microscope please contact Dr. Pei Wang of the Wang Lab.
Seahorse XFe Extracellular Flux Analyzer
The Extracellular Flux Analyzer measures mitochondrial respiration and glycolysis in live cells. The technology uses solid-state sensors to simultaneously measure both oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) in either 24-well or 96-well plate format. All platforms support injection of up to 4 unique compounds per well.
The XFe96 Seahorse Analyzer is available as part of the Cell Function Analysis Core under Diabetes Research Center. Please contact the Director Sakeneh Zraika or Associate Director Rong Tian for more details.
If you wish to access the XFe24 Seahorse Analyzer please contact Dr. Toni Hsu of the Tian Lab.
Fluorescent Microscope
The QuantaMaster800 is a high-speed multi-wavelength fluorescence system and has capability to switch excitation wavelengths at a speed of a millisecond, maximize dynamic range of fluorescence probes, determine ideal wavelengths for the fluorescence probes, acquire highest quality results in challenging multiple-probe experiments, measure fast transients (up to 250 ratios per second), and perform ion intracellular measurements.
If you wish to access the Fluorescent Microscope please contact Dr. Toni Hsu of the Tian Lab.
