Broadly, my research interests are in the area of metabolomics, both methods development and applications focusing investigations of human health and diseases. Methods development, utilizing nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MS) techniques, is focused on the analysis of metabolites in complex biological mixtures from humans, animal models and cell lines.

Some of the current research projects are:

• Development of NMR methods for analysis of the notoriously unstable but critically important metabolites that are fundamental to cellular functions.
• Unknown metabolite identification and extending the limit of metabolite quantitation using NMR.
• Investigation of metabolism in live cells and subcellular organelles using real time NMR.
• Using isotope labeled tracers for investigation of metabolic pathways.
• Development of methods for the assessment of pre-analytical sample quality in routine metabolomics applications.
• Development of quantitation methods using NMR.
• Development of NMR-guided MS methods for analysis of unstable and stable metabolites.
• Methods to exploit the combined strength of NMR and MS in metabolomics.

Education

BS Mysore University, Chitradurga, India. 1983
MS Mysore University, Mysore, India, 1985
PhD Bangalore University, Bangalore, India, 1999
Postdoctoral Scientist   State University of New York, Buffalo, NY, USA, 1998

Nagana Gowda GA, Yashas GN, Raftery D. Expanding the Limits of Blood Metabolite Quantitation using NMR Spectroscopy. Anal. Chem. 87(1), 706-715, 2015.

Nagana Gowda GA, Yashas GN, Raftery D. Massive cyclization of glutamine to pyroglutamic acid discovered using NMR spectroscopy, Anal. Chem., 87(7), 3800-3805, 2015.

Nagana Gowda GA, Raftery D. Can NMR solve some significant challenges in metabolomics? J Magn Reson. 260:144-60, 2015.

Tayyari F, Nagana Gowda GA, Gu H, Raftery D. ¹⁵N-cholamine–a smart isotope tag for combining NMR- and MS-based metabolite profiling. Anal Chem. 85(18):8715-21, 2013.

Appiah-Amponsah E, Owusu-Sarfo K, Nagana Gowda GA, Ye T, Raftery D. Combining Hydrophilic Interaction Chromatography (HILIC) and Isotope Tagging for Off-Line LC-NMR Applications in Metabolite Analysis. Metabolites. 3(3):575-91, 2013