The Fujise Laboratory
The Lab is located on the 3rd floor of the Brotman Building. It occupies roughly 1500 square feet, in addition to a tissue culture suite and office space. The Brotman Building is one of six research buildings on the University of Washington (UW) South Lake Union (SLU) campus. Members of this laboratory have full access to a pool of shared equipment and core laboratories of both SLU and other UW campuses.
Institutional Scientific Culture and Intellectual Environment
University of Washington
Founded in 1861, UW has enjoyed the reputation of being a top research university and the professional home to a number of outstanding alumni and faculty, including 20 Nobel laureates. Researchers at UW are supported by over $1 billion in research funding annually. As such, the university’s 700-acre campus with 500+ buildings and 20+ million gross square feet space provides its faculty, students, and staff with a remarkably rich intellectual environment for scientific research. UW and its research leadership are committed to biomedical research and have robustly invested in and diligently maintained the research infrastructure. UW hosts cross-disciplinary courses, seminars, and training sessions for faculty members, postdoctoral fellows, staff scientists, and graduate students on a daily basis.
UW SLU Campus
The SLU campus encompasses six interconnected buildings and houses about 50 principal investigators from the Department of Medicine, thereby representing an exceptionally interactive scientific environment. Scientific conferences are held somewhere on campus daily, and visiting professors are welcomed on a weekly basis. The Fujise Lab is well positioned to conduct and successfully complete its projects. The facilities available within the UW campuses, together with the positive and collaborative culture that the Lab has created within the lab as well as with all of the collaborators, will allow the team to achieve the goals of various ongoing projects.
UW SLU Core Research Facilities
They provide the investigators with exceptional research infrastructure and include the following facilities within the SLU campus of the UW:
- High Resolution Imaging and Spectroscopy Core Facility is equipped with 14 Tesla (600MHz) vertical wide bore Bruker Magnetic Resonance Spectrometers and others and is capable of multi-nuclear, high resolution NMR as well as in vivo MRS and micro MRI.
Visit MMC Site - Vision Core Lab Transmission Electron Microscope is located on the SLU campus and belongs to the Department of Ophthalmology within UW Medicine; it is equipped with a JEOL 1230 Transmission Electron Microscope with an ATM digital camera. Also available is a Leica Ultramicrotome.
Visit Vision Core Lab - Bio-Molecular Imaging Center is equipped with a state-of-the-art 3T Philips Ingenia MRI. This research-only MRI center provides research MRI services for the UW and the Puget Sound Region. The Center also offers CardiacQuant, a package of new applications for cardiology allowing the investigators to noninvasively assess myocardial tissue characteristics; MultiBand SENSE, which enables fMRI and DTI exams with high speed and high resolution, is also available.
BMIC Website - Flow and Imaging Cytometry Core Lab is equipped with three FACS Aria cell sorters, the Cytek Aurora, the LSR II, the FACS Canto II, the Canto RUO, and the Amnis Imagestream X Mark II. This lab provides a broad range of instrumentation and expertise for addressing experimental challenges related to single cells.
Visit Flow Lab Website - Quellos High-throughput Screening Core was established by a charitable gift from the Quellos Group. It is equipped with essential instrumentation and equipment that makes high-throughput screening possible. The Core performs cancer drug sensitivity tests, chemical screening against cells or classical biochemical targets, and RNA interference or loss of function screening.
Quellos High-throughput Screening Core Webpage - Histology and Imaging Core (HIC) provides efficient, high-quality solutions for research needs including immunohistochemistry (Leica Bond Rx, Leica Bond MAX), image analysis (Tissue-Tek VIP, Leica EG1150H, Leica RM2255, Leica CM1950, Leica Autostainer XL), digital microscopy (Hamamatsu Nanozoomer While Slide Scanner, DeltaVision Elite Integrated High-Resolution Fluorescence Microscopy Platform, Nikon Eclipse 90i Advanced Research Microscope), histology (Visiopharm Quantitative Digital Pathology), multiplex immunoassay (Luminex Bio-Plex 200® systems), and comparative pathology consultation.
HIC Website - Tom & Sue Ellison Stem Cell Core was made possible by a generous gift from Tom and Sue Ellison. This facility aims to promote the use of pluripotent stem cells within the Seattle biomedical community and provides the UW and other scientists with the following services: iPSC generation, mouse ESC generation, teratoma creation, gene editing, distribution of pluripotent cell stocks, and gamma-irradiation of rodents for bone-marrow transplantation.
Tom & Sue Ellison Stem Cell Core Webpage - Northwest Metabolomics Research Center is focused on brining together, coordinating, and integrating significant capabilities in metabolomics and offers (i) mass spectrometry (SCIEX Triple Quad 6500+/Shimazu Nexera 20XR HPLC; SCIEX Qtrap 5500 MS/Shimazu LC-30 HPLC; Waters Xevo TQ-S Micro MS; Agilent 6410 TQMS/Agilent 1260 HPLC; Agilent 6520 Q-TOF/Agilent 1200 LC; Agilent 7890A/5875 GC-MS), (ii) NMR (Bruker 800&700 MHz NMR), and (iii) statistics and bioinformatics services.
NW Metabolomics Website - Pathology Research Services Laboratory offers morphologic techniques including and not limited to paraffin, frozen and electron microscopy tissue processing and embedding, routine histology, tissue enzyme histochemistry, immunohistochemistry, immunofluorescence, TUNEL staining, digital photomicrography, and computerized quantitative morphometric analysis.
Pathology Research Services Laboratory Webpage - SLU Small Animal Imaging Center is equipped with the IVIS Spectrum In Vivo Imaging System that is capable of performing optical in vivo imaging of bioluminescence and fluorescence in small animals. Data acquisition is conducted using Living Image software.
SLU Imagining Center Webpage